la taq pcr enzyme (TaKaRa)

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TaKaRa la taq pcr enzyme
La Taq Pcr Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/la+taq+enzyme/TaKaRa+LA+Taq+DNA+Polymerase+with+GC+Buffer/pm41599334-61-6-10
Average 95 stars, based on 262 article reviews

la taq pcr enzyme - by Bioz Stars, 2026-09

95/100 stars

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Polymerase Chain Reaction:

Article Title: Identification of genes related to agarwood formation: transcriptome analysis of healthy and wounded tissues of Aquilaria sinensis
Article Snippet: The mRNA was reverse-transcribed with PowerScript II (Takara, Japan) using SMART IV oligonucleotide PCR primers (5 ′ -AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3 ′ ) and a CDS III/3 ′ PCR primer (5 ′ -ATTCTAGAGGCCGAGCGGCCGACATG -d[T]30 N-1 N-3 ′ ). .. Long-distance PCR for double-stranded cDNA amplification was performed with LA Taq enzyme (Takara, Japan) for 25 cycles (95°C for 30 s, 68°C for 8 min) according to the SMART cDNA Library Construction Kit user manual. .. Finally, the double-stranded cDNA was purified using a DNA purification kit (Qiagen, Germany) to generate high-quality cDNA.

Article Title: Red Fluorescent Protein Variant with a Dual-Peak Emission of Fluorescence.
Article Snippet: The marine environment is a rich reservoir of diverse biological entities, many of which possess unique properties that are of immense value to biotechnological applications.. One such example is the red fluorescent protein derived from the coral Discosoma sp. This protein, encoded by the DsRed gene, has been the subject of extensive research due to its potential applications in various fields.. In the study, a variant of the red fluorescent protein was generated through random mutagenesis using the DsRed2 gene as a template.

Article Title: Molecular survey on vector-borne pathogens in clinically healthy stray cats in Zaragoza (Spain).
Article Snippet: .. The cPCR cycling conditions consisted of an initial denaturation step at 94 °C for 2 min, followed by 40 cycles of 94 °C for 15 s, 57 °C for 15 s and 72 °C for 15 s. All cPCR assays were performed in a final volume of 25 μl that contained 2 μl of DNA extract, 2.5 μl of 10 × TaKaRa LA Taq buffer, 0.25 μl of TaKaRa LA Taq enzyme (Takara Bio Europe S.A.S.Saint-Germain-en-Laye, France), 2.5 μl of MgCl2 (25 mM), 1 μl each of forward and reverse primers (50 μM), 4 μl of dNTPs and PCR grade water up to 25 μl. .. For species and genotype characterization, the obtained ITS cPCR products were sequenced in both directions using BigDye 3.1 Ready Reaction Mix (Applied Biosystems) according to the manufacturer’s instructions.

Article Title: Evaluating the impact of short educational videos on the cortical networks for mathematics.
Article Snippet: The cDNA was synthesized from isolated RNA by using a PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TaKaRa, China), following the manufacturer’s protocol, and used for PCR-amplification with the primer pair (uch-CDS) based on the annotated M. virgata USP15 gene (MvUSP15 gene) from the M. virgata genome (Supplementary Table 5). .. PCR reactions using LA Taq enzyme (TaKaRa, China) were performed on the thermal cycler (Biometra) with an initial denaturation step of 94 °C for 3 min, followed by 39 amplification cycles consisting of denaturation at 94 °C for 30 s, annealing at 51 °C for 30 s, elongation at 72 °C for 2 min and 30 s, and the reactions were terminated with an elongation step of 72 °C for 10 min, followed by cooling at 4 °C. .. The amplified products were first purified using an AxyPrepTM DNA Gel Extraction Kit (Corning, China), followed by an independent ligation process into the pMD19-T Vector system (TaKaRa, China).

Article Title: Genome-wide sequencing identifies a thermal-tolerance related synonymous mutation in the mussel, Mytilisepta virgata
Article Snippet: The cDNA was synthesized from isolated RNA by using a PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TaKaRa, China), following the manufacturer’s protocol, and used for PCR-amplification with the primer pair (uch-CDS) based on the annotated M. virgata USP15 gene ( MvUSP15 gene) from the M. virgata genome (Supplementary Table ). .. PCR reactions using LA Taq enzyme (TaKaRa, China) were performed on the thermal cycler (Biometra) with an initial denaturation step of 94 °C for 3 min, followed by 39 amplification cycles consisting of denaturation at 94 °C for 30 s, annealing at 51 °C for 30 s, elongation at 72 °C for 2 min and 30 s, and the reactions were terminated with an elongation step of 72 °C for 10 min, followed by cooling at 4 °C. .. The amplified products were first purified using an AxyPrepTM DNA Gel Extraction Kit (Corning, China), followed by an independent ligation process into the pMD19-T Vector system (TaKaRa, China).

Amplification:

Article Title: De Novo Transcriptome Profiling for the Generation and Validation of Microsatellite Markers, Transcription Factors, and Database Development for Andrographis paniculata .
Article Snippet: For the cDNA library preparation enriched mRNAs were reverse-transcribed by PowerscriptTM II (Takara, Tokyo, Japan) with PCR primers SMART IVTM Oligonucleotide (5′-AAGCAGTGGTATINOSPORAAACGCAGAGTGGCCATTACGGCCGGG-3′) and CDS III/3′ PCR Primer (5′-ATTINOSPORATAGAGGCCGAGGCGGCCGAC ATG-d (T) 30N1N-3′) (Clonetech, Mountain View, CA, USA). .. Next, for double-strand cDNA amplification, long-range PCR was performed using the LA Taq enzyme (Takara, Japan) for 25 cycles (95 ◦C for 30 s, 68 ◦C for 8 min) according to the SMARTTM cDNA Library Construction Kit. .. Finally, to produce high-quality cDNAs, double-stranded cDNAs were purified using a DNA purification kit (Qiagen, Hilden, Germany).

Article Title: The exploration of genetic aetiology and diagnostic strategy for 321 Chinese individuals with intellectual disability.
Article Snippet: Background: Intellectual disability is a heterogeneous neurodevelopmental disorder with complex genetic architectures.. Different sequential methodologies are usually applied to identify the genetic aetiologies of ID patients.. Methods: We collected 321 consecutive ID patients.

cDNA Library Assay:

Long Range PCR:

Concentration Assay:

Article Title: Foot-and-mouth disease virus carrier status in Bos grunniens yaks
Article Snippet: .. The final concentration of each reagent was: 0.4 mM dNTP, 0.4 μM forward primer, 0.4 μM reverse primer, cDNA 6 μl, 10 × LA Buffer 5 μl, and LA Taq enzyme 0.05 U/μl (TaKaRa). .. The RT-PCR products of VP1 were purified with Gel DNA Extraction Kit (QIAGEN).

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